| Star Product |
Yes |
| REF No |
5110 |
| Trademark |
GBL® ROSA-STAIN |
| Product Name |
WEB İÇİN |
| Regulation |
|
| Purpose |
Congo Red Highman is a histological staining method used specifically to detect amyloid deposits. |
| Working Principle
|
Congo Red: Congo red is an aniline dye that has a tendency to bind to amyloid proteins. This binding allows amyloids to be clearly visible under the microscope. Sections are incubated in Congo red solution for a specific time. In this step, Congo red binds to amyloid fibrils, causing the amyloid deposits to appear red. |
| Warnings, Precautions, Recommendations
|
Danger. Warning. Highly flammable liquid and vapour. Causes serious eye irritation. May cause cancer. Keep away from heat/sparks/flames/hot surfaces. - No smoking. Keep container tightly closed. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Dry skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. |
| Ingredients
|
A) Congo Red Solution: Ethanol 96%, Congo red
B) Alkaline Solution: Ethanol 96%, Potassium buffers
C) Phosphate Buffer Solution: Phosphate buffers
D) Mayer Hemalum Solution: Hematoxylin, Aluminum ammonium sulfate, Glycerol |
| Colour
|
|
| Odour
|
|
| Storage Temperature
|
15-25 ℃ |
| Shelf Life After Opening the Lid
|
|
| Shelf Life
|
2 years |
| pH (at 25°C)
|
|
| Appearance
|
|
| Concentration
|
Congo Red Highman Staining, Congo Red Staining, Amyloid Staining |
| Accessories
|
|
| Application
|
1. Cover the preparation with 10 drops of “A- Congo Red Solution”.
2. After 15 minutes, shake the slide to remove the reagent.
3. Wash with distilled water for 1 minute.
4. Cover the preparation with 10 drops of “B- Alkaline Solution”.
5. After 30 seconds, shake the reagent
6. Wash with distilled water for 5 minutes.
7. Cover the preparation with 10 drops of “C- Phosphate Buffer Solution”.
8. After 2 minutes, shake the slide to remove the reagent.
9. Cover with 10 drops of “D- Mayer Hemalum Solution” without washing.
10. After 5 minutes, shake the slide to remove the reagent.
11. Wash with distilled water for 5 minutes.
12. Dehydrate, clean, cover with synthetic resin and examine under a microscope. |
| Microbiological Properties
|
|
Amyloid |
From orange to red |
|
Nucleus |
Blue |
|
Back areas |
Colorless |
|
Eosinophil granules |
Red |
|
Elastic fibers |
Light red to red |
|
Amyloid (in polarized light) |
Green metachromasia |
|
| Ordering and Packaging Information
|
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in Box |
|
445110050050 |
Piece |
50 mL |
50 ml, HDPE White Bottle with Dropper |
1 set |
|