Title

Description

Star Product

Yes

REF No

5029

Trademark

GBL® ROSA-STAIN

Product Name

WEB İÇİN

Purpose

Used for light microscopic visualization of acid mucopolysaccharides, glycogen, glycoprotein, mucoprotein, high molecular weight carbohydrates in tissue sections. Specially designed for use in formalin-fixed paraffin block tissue sections or cytologic preparations for routine tissue follow-up.

Working Principle

Alcyan blue is a water-soluble polyvalent basic dye. Its blue staining property comes from the copper in the molecule. At acid pH formed by acetic acid, a) sulfated acid mucopolysaccharides, b) carboxylated acid mucopolysaccharides, c) sulfated sialomucins (glycoproteins), d) carboxylated sialomucins (glycoproteins) stain with alcyan blue. Periodic acid selectively oxidizes 1,2-glycolic, primary amine, secondary amine and hydroxy ketonic groups. High molecular weight carbohydrates carry these groups. As a result of oxidation, the ends of some carbon atoms in the structure become free. It retains leucofucsin dye (sulfurized fucsin dye) and prevents it from dissolving in water. The parts where the dye is retained appear magenta red.

Warnings, Precautions, Recommendations

Caution. Irritant. Toxic. May cause cancer. In contact with acids, releases toxic gas. Prolonged or repeated exposure may cause allergic reactions in some sensitive individuals. Irritating to eyes and skin, releases toxic gas on contact with acids. Wear protective gloves/protective clothing/eye protection/face protection. IN CASE OF CONTACT WITH SKIN: Wash with plenty of soap and water. In case of inhalation: Shortness of breath, mucosal irritation, cough, headache in case of prolonged exposure. In case of skin irritation: Get medical advice/attention. In case of ingestion: Consult a doctor.

Ingredients

A)Alcyan Blue pH: 2,5 solution: Alcyan blue 8GX, Acetic acid
B) Borate Buffer Solution: Boric acid, Sodium tetraborate
C) Periodic Acid Solution: Periodic acid
D) Schiff Separator McManus: Pararosaniline, Sodium metabisulfite, Hydrochloric acid
E) Sodium Metabisulfite Solution: Sodium metabisulfite
F) Detection Solution: Sodium metabisulphite, Hydrochloric acid
G) Mayer Hemalum Solution: Ethanol 96%, Hematoxylin, Glycerol, Aluminum ammonium sulfate

Storage Temperature

2-8 °C

Shelf Life

2 years

Concentration

PAS Staining, Alcyan Blue PAS

Application

1. Cover the tissue section with 10 drops of “A- Alcyan Blue pH: 2.5 solution”. After 30 minutes, remove the reagent by shaking the slide.
2. Cover with 10 drops of “B- Borate Buffer Solution” without washing with water. After 10 minutes, shake the slide to remove the reagent.
3. Wash with distilled water for 2 minutes.
4. Cover the tissue section with 10 drops of “C- Periodic Acid Solution”. After 10 minutes, shake the slide to remove the reagent.
5. Wash with distilled water for 2 minutes.
6. Cover with 10 drops of “D- Schiff's Separator McManus”. After 20 minutes, shake the slide to remove the reagent.
7. Wash with distilled water for 2 minutes.
8. Cover with 10 drops of “E- Sodium Metabisulphite Solution”. After 2 minutes, shake the slide to remove the reagent.
9. Cover with 10 drops of “F- Fixing Solution” without washing with water. After 2 minutes, shake the slide to remove the reagent. Shorten or remove this step for significant PAS positive reaction.
10. Wash with distilled water for 2 minutes.
11. Cover with 10 drops of “G- Mayer Hemalum Solution”. After 3 minutes, shake the slide to remove the reagent.
12. Wash with distilled water for 5 minutes.
13. Dehydrate, clean, cover with synthetic resin and examine under a microscope.

Microbiological Properties

Positive PAS areas	Magenta red
Acid mucopolysaccharides	Turquoise-blue
Nucleus, some epithelial mucins and cartilage	Blue

Ordering and Packaging Information

Order No	Unit	Volume	Packaging Type	Quantity in a Box
445029050050	Piece	50 mL	50 ml, White Bottle with HDPE Dropper	1 kit
445029020500	Piece	500 mL	500 ml, HDPE White Bottle 28 mm	1 kit