| Star Product |
Yes |
| REF No |
5007 |
| Trademark |
GBL® ROSA-STAIN |
| Product Name |
WEB İÇİN |
| Regulation |
|
| Purpose |
It is used to demonstrate Gram (+) and Gram (-) bacteria by light microscopy. |
| Working Principle
|
Gram positive/negative status is a feature that depends on the structure of the bacterial cell wall (peptidoglycan). The peptidoglycan structure of Gram negative bacteria is thinner between the outer membrane and the inner membrane than Gram positive bacteria. In Gram positive bacteria, the thick peptidoglycan has the property of retaining crystal violet. Lugol allows the dye to be retained. The cell wall permeability of Gram positive bacteria is less. Therefore, they do not release the dyes during decolorization with acetone alcohol. In Gram negative bacteria, acetone alcohol dissolves the lipids in the outer membrane, the pores open and the crystal violet disperses. Thus, Gram negative bacteria are stained with safranin added to the medium later. |
| Warnings, Precautions, Recommendations
|
Danger. Highly flammable liquid and vapour. Causes serious eye irritation. Harmful if swallowed. May cause organ damage. Suspected of causing cancer. Keep away from heat/sparks/flames/hot surfaces. Wear protective gloves/protective clothing/eye protection/face protection. IF ON SKIN (or hair): Remove/Take off immediately all contaminated clothing. Dry skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if worn and easy to do. Continue rinsing. IF exposed or concerned: Get medical advice/attention. Store locked up. |
| Ingredients
|
A) Gram Crystal Violet Solution: Ethanol, 96%, Crystal Violet, Di-ammonium oxalate
B) Gram Lugol's Solution: Iodine, Potassium iodide
C) Gram Acetone Bleach Solution: Methanol, Acetone, Ethanol, 96%
D) Gram Safranin Solution: Safranin O, Triton X-100, Ethanol, 96% |
| Colour
|
|
| Odour
|
|
| Storage Temperature
|
15-25°C |
| Shelf Life After Opening the Lid
|
|
| Shelf Life
|
2 years |
| pH (at 25°C)
|
|
| Appearance
|
|
| Concentration
|
Gram-negative bacteria, Gram-positive bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s crystal violet Solution, Gram’s iodine Solution, Gram’s Decolorizer Solution, Gram’s safranin Solution, Gram staining, Gram's method |
| Accessories
|
|
| Application
|
1. Cover the preparation with “A-Gram Crystal Violet Solution”. After 1 minute, shake and pour.
2. Gently wash with distilled water.
3. Cover the preparation with “B-Gram Lugol’s Solution”. After 1 minute, shake and pour.
4. Gently wash with distilled water.
5. Cover the preparation with “B-Gram Acetone Bleach Solution”. After 5-15 seconds, shake the slide to remove the reagent.
6. Gently wash with distilled water.
7. Repeat this process once more.
8. Gently wash with distilled water.
9. Cover the preparation with “D-Gram Safranin Solution”. After 30 seconds, shake the slide to remove the reagent.
10.Wash gently with distilled water.
11.Dry in air, preferably on a heated table. Examine under a microscope. |
| Microbiological Properties
|
|
Gram positive microorganisms |
Bluish purple |
|
Gram negative microorganisms |
Pinkish Red |
|
| Ordering and Packaging Information
|
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445007050250 |
Piece |
250 mL |
250 ml, PP White Bottle with Dropper |
1 kit |
|