Star Product |
Yes |
REF No |
2109 |
Trademark |
GBL® ROSA-STAIN |
Product Name |
WEB İÇİN |
Regulation |
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Purpose |
It is used in microbiology and clinical diagnostics, especially for the detection of acid-resistant bacteria (ARB) such as Mycobacterium tuberculosis. The purpose of this solution is to allow such bacteria to accumulate in the cell walls and to be seen brightly under a fluorescence microscope. |
Working Principle
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It is a staining technique used to visualize acid-fast bacilli using fluorescence microscopy. Acid-fast organisms exhibit a reddish yellow fluorescence color. Although the auramine-rodamine stain is not as specific as the Ziehl-Neelsen stain for acid-fast organisms (e.g. Mycobacterium tuberculosis or Nocardia), it gives faster results, so is often used as a screening tool. |
Warnings, Precautions, Recommendations
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Ingredients
|
Potassium permanganate |
Colour
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Odour
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Storage Temperature
|
15/25°C |
Shelf Life After Opening the Lid
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Shelf Life
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2 years |
pH (at 25°C)
|
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Appearance
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Concentration
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Potassium Permanganate Solution, Potassium Permanganate, ARB, Acid resistant microorganisms |
Accessories
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Application
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1. Cover with “A-Auramine Rhodamine Solution”. Shake off after 15 minutes.
2. Wash with distilled water.
3. Cover with “B- Acidic Bleaching Solution”. Shake off after 1 minute. You can change the reagent many times during this time.
4. Wash with distilled water.
5. Cover with “C- Potassium Permanganate Solution”. After 3 minutes, shake the preparation to remove the reagent.
6. Wash with running distilled water.
7. Examine under a microscope. |
Microbiological Properties
|
Acid resistant microorganisms |
Yellow to reddish fluorescence |
Back areas |
Black |
|
Ordering and Packaging Information
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
442109020500 |
Piece |
500 mL |
500 ml, HDPE White Bottle 28 mm |
24 |
|