| Star Product |
Yes |
| REF No |
5192 |
| Trademark |
GBL® ROSA-STAIN |
| Product Name |
WEB İÇİN |
| Regulation |
|
| Purpose |
It is used for the demonstration of lipids in fresh tissue sections. In particular, the demonstration of fat cells and neutral lipids is a common use. It can also be used to detect abnormal lipid location or entrainment and to differentiate some tumors with excessive amounts of fat cells from others. |
| Working Principle
|
Oil Red O is a dye that dissolves in lipid tissue. This makes the fatty parts appear red under the microscope. Propylene glycol protects lipids, allowing accurate examination of cell and tissue samples. |
| Warnings, Precautions, Recommendations
|
Danger Harmful Causes severe skin burns and eye damage. May cause respiratory irritation. Suspected to cause cancer. Do not breathe dust/fume/gas/mist/vapor/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove the affected person to fresh air and keep in a comfortable position so that he/she can breathe easily. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. In case of exposure or interaction IF: Get medical advice/attention. If eye irritation persists: Seek medical advice/attention.
Translated with DeepL.com (free version) |
| Ingredients
|
A) Formalin Buffered Neutral Fixative: Formaldehyde, Phosphate buffers
B) Propylene Glycol: Propylene glycol
C) Oil Red O Dye: Oil red O
D) Propylene Glycol 85%: Propylene glycol
E) Hematoxylin Mayer Solution: Hematoxylin, Aluminum ammonium sulfate, Sodium iodate, Stabilizer
F) Sodium Phosphate Solution: Phosphate buffers, condoms
G) Covering Medium; Glycerol: Gelatin, Phenol, Glycerol |
| Colour
|
|
| Odour
|
|
| Storage Temperature
|
2-8 °C |
| Shelf Life After Opening the Lid
|
|
| Shelf Life
|
2 years |
| pH (at 25°C)
|
|
| Appearance
|
|
| Concentration
|
Lipid Preservation Staining Kit with Propylene Glycol, Propylene Glycol Lipid Stain |
| Accessories
|
|
| Application
|
1.Air dry the preparation at room temperature for 30-60 minutes.
2. Immerse in a container of “A-Formalin Buffered Neutral Fixative”. After 10 minutes, shake off.
3.Wash quickly 3 cycles with distilled water.
4.Allow to air dry for a few more minutes.
5. Immerse in a container of “B-Propylene Glycol”. After 5 minutes, shake it out.
6. Immerse in a container of “C- Oil Red O Dye” and leave at 60-65°C for 10 minutes. Shake off at the end of the time. Use a hot water bath to create this temperature. Filter if precipitation is present despite standing at this temperature for a certain period of time.
7. Immerse in a container of “D- Propylene Glycol Solution 85%”. After 5 minutes, shake off.
8. Wash 2 cycles with distilled water.
9. Immerse in a container of “E- Hematoxylin Mayer's Solution”. Shake off after 1 minute.
10. Wash with tap water for 3 minutes.
11. Immerse in a container of “F- Sodium phosphate solution”. Shake off after 1 minute.
12.Wash with distilled water.
13.Cover with “G-Covering Medium with Glycerol” which you have stored at 2-8°C and melted in a 60°C water bath for 5 minutes before use. |
| Microbiological Properties
|
|
| Ordering and Packaging Information
|
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in Box |
|
445192030050 |
Piece |
50 mL |
50 ml, HDPE White Bottle with Dropper |
1 set |
|