2424-Gram Fuchsin Solution

Title Description
Star Product Yes
REF No 2424
Trademark GBL® ROSA-STAIN
Product Name WEB İÇİN
Regulation
Purpose It is the counting stain of the Gram staining method. With this staining procedure, microorganisms are separated as gram positive and gram negative.
Working Principle Gram Positive/Negative status is a feature of the cell wall (peptidoglycan) structure of bacteria. The peptidoglycan structure of Gram Negative bacteria is thinner between the outer membrane and inner membrane than Gram Positive bacteria. In Gram positive bacteria, the thick peptidoglycan is capable of retaining crystal violet. Lugol ensures the retention of the dye. Cell wall permeability of Gram positive bacteria is less. Therefore, they do not release dyes during decolourisation with denatured alcohol. In Gram-negative bacteria, alcohol dissolves the lipids in the outer membrane, the pores open and the crystal violet disperses. Gram-negative bacteria are then stained with fuchsin added to the medium.
Warnings, Precautions, Recommendations Highly flammable liquid and vapour.
Ingredients Fuchsin, phenol, ethanol.
Colour
Odour
Storage Temperature 15-25°C
Shelf Life After Opening the Lid 6 months
Shelf Life 24 months
pH (at 25°C) 5,6±0,3
Appearance
Concentration Gram-negative bacteria, Gram-positive bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s crystal violet Solution, Gram’s iodine Solution, Gram’s Decolorizer Solution, Gram’s safranin Solution, Gram staining, Gram's method.
Accessories
Application 1)If the smear is too acidic, cover with Sodium Hydrogen Carbonate Solution for 15-30 seconds.
2)Cover the smear with Crystal Violet Stain (GBL-2434) for 1-2 minutes.
3)Wash with distilled water.
4)Cover the smear with Gram Lugol's Solution (GBL-2435) for 1 minute.
5)Washed with distilled water.
6)Cover the smear with Denatured Alcohol (GBL-2891) for 5-15 seconds.
7)Washed with distilled water.
8)Cover the smear with Gram Fuchsin Aqueous Stain (GBL-2424) for 1 minute.
9)Gently washed with distilled water. Air dried and analysed.
Microbiological Properties
Gram positive bacteria Blue-black
Gram negative bacteria Red

Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Parcel
442424020500 Bottle 500 mL 500 ml, HDPE White Bottle 24

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