2217-Eosin Nigrosin Sperm Stain

Title Description
Star Product Yes
REF No 2217
Trademark GBL® FERTI-ROSE
Product Name WEB İÇİN
Regulation
Purpose It is used to determine the number of live sperm when sperm motility is low.
Working Principle The basis of eosin nigrosin sperm staining is based on the principle that dead sperm take the dye in and appear stained because their membrane structure is disrupted.
Warnings, Precautions, Recommendations Caution. Causes serious eye irritation. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. If eye irritation persists: Seek medical advice/help. Wear protective gloves/protective clothing/eye protection/face protection.
Ingredients Sodium Chloride, Eosin Y, Nigrosin, Stabilizers.
Colour
Odour
Storage Temperature 2/8 °C
Shelf Life After Opening the Lid
Shelf Life 1 year.
pH (at 25°C) pH: 7,0±0,2
Appearance
Concentration Eosin Nigrosin Sperm Stain
Accessories
Application 1. Mix the semen sample thoroughly.
2. Mix 0.05 ml of semen sample and 0.05 ml of Eosin-Nigrosin stain in a test tube and wait 30 seconds.
(1 drop = 0.05 ml)
3. After 30 seconds, prepare a smear preparation on a slide by taking 1 drop of the suspension and allow to air dry.
4. Fix in water-free fixative medium.
5. Examine under a bright-field microscope at x1000 magnification and immersion oil.
6. Count stained (dead) or unstained (live) cells with a laboratory cell counter.
Microbiological Properties
Live spermatozoa Colorless
Dead spermatozoa Pink - Red

Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
442217050050 Quantity 50 ml 50 ml, HDPE White Bottle with Dropper 1 Quantity

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