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GBL.COM.TR
2423-Ziehl Neelsen Carbol Fuchsin Stain
Title
Description
Star Product
Yes
REF No
2423
Trademark
CONVASTAIN
Product Name
WEB İÇİN
Regulation
Purpose
Designed for general laboratory use. For the Ziehl Neelsen acid-fast staining method. Divides microorganisms according to whether they are acid-resistant or not.
Working Principle
Acid-resistant organisms such as Mycobacterium tuberculosis contain large amounts of lipid substances called mycolic acids in their cell walls. These acids are resistant to staining by ordinary methods such as Gram staining.
The reagents used in Ziehl-Neelsen staining are carbol fuchsin, acid alcohol and methylene blue. Acid-resistant bacilli (ARB) appear bright red after staining.
Warnings, Precautions, Recommendations
Highly flammable liquid and vapour. Harmful if swallowed. Causes severe skin burns and eye damage. Suspected of causing genetic damage.
Ingredients
Fuchsin, phenol, ethanol.
Colour
Odour
Storage Temperature
15/25 °C
Shelf Life After Opening the Lid
Right away
Shelf Life
24 months
pH (at 25°C)
Appearance
Concentration
acid-fast stain, Ziehl-Neelsen stain bacteria, asidofilik, Ziehl Neelsen, asit-fast, carbol-fuchsin, aside dirençliler, ZN,
Accessories
Application
-The smear is stained with Carbol Fuchsin Stain (GBL-2423) by heating from the bottom for 5 minutes.
-Cooled. Wash with distilled water for 5-10 seconds.
-Cover the smear with Acid Alcohol (GBL-2426) for 5-30 seconds.
-Gently washed very briefly with distilled water.
-The smear is stained with Methylene Blue Alkali Stain, Löffler (GBL-2420) for 1-2 minutes.
-Wash gently with distilled water. Air-dried and analysed.
Microbiological Properties
Acid-resistant microorganisms
Bright Red
Others and back field elements
Blue
Ordering and Packaging Information
Order No
Unit
Volume
Packaging Type
Quantity in a Parcel
442423050250
Bottle
250 ml
250 ml, PP White Bottle with Dropper
32
442423020500
Bottle
500 ml
500 ml, HDPE White Bottle
24
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