5192-Oil Preservative Lipid Staining Kit; Propylene Glycol

Title Description
Star Product Yes
REF No 5192
Trademark GBL® ROSA-STAIN
Product Name WEB İÇİN
Regulation
Purpose It is used for the demonstration of lipids in fresh tissue sections. In particular, the demonstration of fat cells and neutral lipids is a common use. It can also be used to detect abnormal lipid location or entrainment and to differentiate some tumors with excessive amounts of fat cells from others.
Working Principle Oil Red O is a dye that dissolves in lipid tissue. This makes the fatty parts appear red under the microscope. Propylene glycol protects lipids, allowing accurate examination of cell and tissue samples.
Warnings, Precautions, Recommendations Danger Harmful Causes severe skin burns and eye damage. May cause respiratory irritation. Suspected to cause cancer. Do not breathe dust/fume/gas/mist/vapor/spray. Wear protective gloves/protective clothing/eye protection/face protection. IF IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Rinse skin with water/shower. IF INHALED: Remove the affected person to fresh air and keep in a comfortable position so that he/she can breathe easily. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. In case of exposure or interaction IF: Get medical advice/attention. If eye irritation persists: Seek medical advice/attention.

Translated with DeepL.com (free version)
Ingredients A) Formalin Buffered Neutral Fixative: Formaldehyde, Phosphate buffers
B) Propylene Glycol: Propylene glycol
C) Oil Red O Dye: Oil red O
D) Propylene Glycol 85%: Propylene glycol
E) Hematoxylin Mayer Solution: Hematoxylin, Aluminum ammonium sulfate, Sodium iodate, Stabilizer
F) Sodium Phosphate Solution: Phosphate buffers, condoms
G) Covering Medium; Glycerol: Gelatin, Phenol, Glycerol
Colour
Odour
Storage Temperature 2-8 °C
Shelf Life After Opening the Lid
Shelf Life 2 years
pH (at 25°C)
Appearance
Concentration Lipid Preservation Staining Kit with Propylene Glycol, Propylene Glycol Lipid Stain
Accessories
Application 1.Air dry the preparation at room temperature for 30-60 minutes.
2. Immerse in a container of “A-Formalin Buffered Neutral Fixative”. After 10 minutes, shake off.
3.Wash quickly 3 cycles with distilled water.
4.Allow to air dry for a few more minutes.
5. Immerse in a container of “B-Propylene Glycol”. After 5 minutes, shake it out.
6. Immerse in a container of “C- Oil Red O Dye” and leave at 60-65°C for 10 minutes. Shake off at the end of the time. Use a hot water bath to create this temperature. Filter if precipitation is present despite standing at this temperature for a certain period of time.
7. Immerse in a container of “D- Propylene Glycol Solution 85%”. After 5 minutes, shake off.
8. Wash 2 cycles with distilled water.
9. Immerse in a container of “E- Hematoxylin Mayer's Solution”. Shake off after 1 minute.
10. Wash with tap water for 3 minutes.
11. Immerse in a container of “F- Sodium phosphate solution”. Shake off after 1 minute.
12.Wash with distilled water.
13.Cover with “G-Covering Medium with Glycerol” which you have stored at 2-8°C and melted in a 60°C water bath for 5 minutes before use.
Microbiological Properties
Lipid Red
Nucleus Blue

Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
445192030050 Piece 50 mL 50 ml, HDPE White Bottle with Dropper 1 set

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