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GBL.COM.TR
5118-Truant Auramine Rhodamine Acid Resistant Fluorescent Staining Kit
Title
Description
Star Product
Yes
REF No
5118
Trademark
GBL® ROSA-STAIN
Product Name
WEB İÇİN
Regulation
Purpose
It is used under the microscope to demonstrate acid-fast bacteria, especially Mycobacterium tuberculosis.
Working Principle
A staining technique used to visualize acid-fast bacilli using fluorescence microscopy. Acid-fast organisms exhibit a reddish-yellow fluorescence color. Although the auramine-rhodamine stain is not as specific as the Ziehl-Neelsen stain for acid-fast organisms (e.g., Mycobacterium tuberculosis or Nocardia ), it gives more rapid results and is therefore often used as a screening tool.
Warnings, Precautions, Recommendations
Danger. Highly flammable liquid and vapour, Keep away from heat/sparks/flames/hot surfaces, No smoking. May cause severe skin burns and eye damage. May cause drowsiness or dizziness. May cause genetic defects. Wear personal protective equipment. IF SWALLOWED: Rinse mouth. DO NOT induce vomiting. IF ON SKIN (or hair): Remove/take off immediately all contaminated clothing. Rinse skin with water/shower. IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Store locked up.
Ingredients
A) Auramine Rhodamine Solution: Auramine o, Rhodamine B, Glycerol, Phenol, Propanol.
B) Acidic Bleach Solution: Propanol, Hydrochloric acid.
C) Potassium Permanganate Solution: Potassium permanganate.
Colour
Odour
Storage Temperature
15-25°C
Shelf Life After Opening the Lid
Shelf Life
2 years
pH (at 25°C)
Appearance
Concentration
acid-resistant fluorescent staining, Acid Resistant stain, Acid-resistant microorganisms
Accessories
Application
1. Cover with “A- Auramine Rhodamine Solution”. Shake off after 15 minutes.
2. Wash with distilled water.
3. Cover with “B- Acidic Bleach Solution”. Shake off after 1 minute. You can change the reagent several times during this period.
4. Wash with distilled water.
5. Cover with “C- Potassium Permanganate Solution”. Shake off the reagent after 3 minutes.
6. Wash with running distilled water.
7. Examine under a microscope.
Microbiological Properties
Acid-resistant microorganisms
Fluorescence ranging from yellow to reddish
Background areas
Black
Ordering and Packaging Information
Order No
Unit
Volume
Packaging Type
Quantity in a Box
445118050250
Piece
250 mL
250 ml, PP White Bottle with Dropper
1 kit
445118050500
Piece
500 mL
500 ml, HDPE White Bottle 28 mm
1 kit
export@gbl.com.tr
+90 533 775 75 58
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