| Star Product |
Yes |
| REF No |
5018 |
| Trademark |
GBL® ROSA-STAIN |
| Product Name |
WEB İÇİN |
| Regulation |
|
| Purpose |
Used for microscopic examination of Gram positive (+) and Gram negative (-) bacteria. |
| Working Principle
|
Gram Positive/Negative status is a characteristic of the cell wall (peptidoglycan) structure of bacteria. The peptidoglycan structure of Gram Negative bacteria is thinner between the outer membrane and the inner membrane than Gram Positive bacteria. In Gram positive bacteria, the thick peptidoglycan is capable of retaining Hucker crystal violet. Burke's iodine solution allows the dye to be retained. Gram positive bacteria have less cell wall permeability. Therefore, they do not release dyes during decolorization with acetone denatured alcohol. In Gram-negative bacteria, alcohol dissolves the lipids in the outer membrane, the pores open and the Hucker crystal violet disperses. Gram-negative bacteria are then stained with safranin added to the medium. |
| Warnings, Precautions, Recommendations
|
Hazard Highly flammable liquid and vapor. Causes severe eye irritation. Harmful if swallowed. May cause drowsiness or dizziness. Causes damage to organs. Suspected to cause cancer. Keep away from heat/sparks/flame/hot surfaces. Wear protective gloves/protective clothing/eye protection/face protection. IN CONTACT WITH SKIN (or hair): Immediately remove all contaminated clothing. Dry skin with water/shower. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing. In case of exposure or interaction IF: Get medical advice/attention. Keep under lock and key. |
| Ingredients
|
A) Hucker crystal violet solution: Crystal violet, Ethanol 96%, Ammonium oxalate
B) Burke's iodine solution: Iodine, Potassium iodide
C) Denatured alcohol solution with acetone: Ethanol 96%, 2-Propanol, Methanol, Acetone
D) Safranin solution: Ethanol 96%, Safranin-O |
| Colour
|
|
| Odour
|
|
| Storage Temperature
|
15-25°C |
| Shelf Life After Opening the Lid
|
|
| Shelf Life
|
2 years |
| pH (at 25°C)
|
|
| Appearance
|
|
| Concentration
|
Gram Bartholomew Staining Set, Gram-negative bacteria, Gram-positive bacteria, differentiation of bacteria, identification of bacterial isolates, staining technique in microbiology, Gram’s crystal violet Solution, Gram’s iodine Solution, Gram’s Decolorizer Solution, Gram’s safranin Solution, Gram staining, Gram's method |
| Accessories
|
|
| Application
|
1. Cover the preparation with “A-Hucker Crystal Violet Solution”. After 1 minute, shake off.
2. Wash with distilled water for 15 seconds.
3. Cover the preparation with “B- Burke's Iodine Solution”. After 1 minute, shake off.
4. Wash with distilled water for 15 seconds.
5. Cover the preparation with “C- Denatured Alcohol Solution with Acetone” After 10 seconds, shake the slide to remove the reagent.
6. Repeat this procedure one more time.
7. Wash with distilled water for 15 seconds.
8. Cover the preparation with “D- Safranin Solution”. After 1 minute, shake the slide to remove the reagent.
9. Wash with distilled water for 15 seconds.
10. Air dry, examine under a microscope. |
| Microbiological Properties
|
|
Gram (+) microorganisms |
Purple |
|
Gram (-) microorganisms |
Red |
|
| Ordering and Packaging Information
|
|
Order No |
Unit |
Volume |
Packaging Type |
Quantity in a Box |
|
445018050250 |
Piece |
250 mL |
250 ml, PP White Bottle with Dropper |
1 kit |
|