7070-Fructose, Photometric Colorimetric

Title Description
Star Product Yes
REF No 7070
Trademark GBL® FERTI-ROSE
Product Name WEB İÇİN
Regulation
Purpose Used for the determination of fructose in spermatozoites.
Working Principle This method is particularly used to determine the amount of fructose in sperm samples because fructose is the energy source of spermatozoites. Fructose concentration is an important indicator for assessing the function and motility of spermatozoites. Low fructose levels may be a sign of dysfunctions in the seminal vesicles or other reproductive system problems. It is an assay for the direct determination of fructose in spermatozoites. The study uses semen from which proteins have been removed by precipitation. It is based on the principle that fructose turns red when heated with hydrochloric acid against resorcin. The fructose level in healthy semen is around 315 mg/dl. Decreases below this figure necessitate the investigation of a pathological event.
Warnings, Precautions, Recommendations Highly flammable liquid and vapor. Keep away from heat/sparks/flame/hot surfaces. - Do not smoke. Causes severe skin burns and eye damage. Wear protective gloves/protective clothing/eye protection/face protection. IN CASE OF CONTACT WITH EYES: Rinse carefully with water for several minutes. Remove contact lenses if fitted and easy to do so. Continue rinsing.
Ingredients A-Barium Hydroxide Solution Barium Hydroxide.
B-Zinc Sulphate Solution Zinc Sulphate.
C-Fructose Stock Standard Solution Benzoic Acid, Fructose.
D-Resorcin Solution Resorcin, Ethanol.
E-Hydrochloric Acid Solution Hydrochloric Acid.
Colour
Odour
Storage Temperature 15/25 °C
Shelf Life After Opening the Lid
Shelf Life 2 year.
pH (at 25°C)
Appearance
Concentration Spermatozoit fruktoz analizi, Seminal sıvı fruktoz ölçümü, Spermatozoa fructose analysis, Sperm function test
Accessories
Application The semen to be examined is allowed to liquefy. Take a test tube. 0.1 ml semen, 2.9 ml distilled water, 0.5 ml A-Barium Hydroxide Solution and 0.5 ml B-Zinc Sulphate Solution are put into the tube respectively. Mix and leave for 3-5 minutes. Centrifuge at high speed. The upper part is separated with a pipette. This prepared solution is the “Test Solution”.
Working Standard:
It should be prepared fresh.
0.1 ml of C-Fructose Stock Standard Solution is taken into a tube. Add 9.9 ml of distilled water. It is mixed. This solution is the “Working Standard” and corresponds to 5% mg (5 mg/dl) fructose.
1) Take 3 test tubes.
2) Mix the tubes properly.
3) Heat the tubes together in a boiling water bath at 90 °C for 10 minutes.
4) Cool all together with running water.
5) Placed in cuvettes. Read at 490 nm against the blind.
Microbiological Properties
Fructose (mg/dl) = (Assay A / Standard A) x 200

Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in Box
457070090040 Quantity

5 ml

50 ml

250 ml
10 ml, HDPE White Bottle with Dropper
50 ml, HDPE White Bottle with Dropper
250 ml, PP White Bottle with Dropper
 1 Set

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