5061-Non-Specific Esterase Leukocyte Staining Kit

Title Description
Star Product Yes
REF No 5061
Trademark GBL® HEMA-ROSE
Product Name WEB İÇİN
Regulation
Purpose It is used to demonstrate alpha-naphthyl acetate esterase activity in leukocytes by light microscopy.
Working Principle Non-specific esterase leukocyte is an enzyme found in the cytoplasm of leukocytes (white blood cells). This enzyme hydrolyzes ester bonds, breaking down esters into their free acid and alcohol components. If leukocytes contain the enzyme esterase, the cells stain as black granulations.
Warnings, Precautions, Recommendations Harmful if swallowed, in contact with skin or inhaled. May be corrosive, irritating.
Ingredients A) Citrate solution pH: 5,4: Sodium chloride, Chloroform, Triton X-100
B) FB blue dye: Fast blue RR salt
C) Sodium nitrite solution: Sodium nitrite
D) Trismalate solution pH: 7.6: Tris, Malic acid, Tween 20
E) Alpha-Naphthyl acetate solution: Alpha-naphthyl acetate
F) Sodium fluoride solution: Sodium fluoride
G) Hematoxylin solution Gill-3: Ethylene Glycol, Hematoxylin, Sodium iodate,
Acetic acid
Colour
Odour
Storage Temperature 2-8 °C
Shelf Life After Opening the Lid
Shelf Life 1 year
pH (at 25°C) D) Trismalate solution pH: 7,6
Appearance
Concentration Non-Specific Esterase Stain, alpha-naphthyl acetate esterase
Accessories
Application 1. Immerse the preparation in the “Fixative solution”. After 30 seconds, shake off the slide and remove the reagent.
2. Wash with distilled water for 1 minute.
3. Immerse the preparation in the “Incubation solution”. Incubate at 37 °C and protected from light. After 30 minutes, remove the slide and shake to remove the reagent.
4. Wash with distilled water for 2 minutes.
5. Stain the preparation with “G- Hematoxylin solution Gill-3” for 2 minutes. At the end of the time, shake the slide to remove the reagent.
6. Wash with distilled water for 15 seconds.
7. Air dry. Examine under a microscope. Use only aqueous covering medium if desired.
Microbiological Properties
Alpha-Naphthyl acetate esterase activity Black granulation

Ordering and Packaging Information
Order No Unit Volume Packaging Type Quantity in a Box
445061050010 Piece 50 mL 50 ml, White Bottle with HDPE Dropper 1 kit

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