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GBL.COM.TR
2408-Giemsa Stain; Concentrate
Title
Description
Star Product
Yes
REF No
2408
Trademark
CONVASTAIN
Product Name
WEB İÇİN
Regulation
Purpose
It is usually used for staining blood cells.
Working Principle
Giemsa stain is a differential stain and contains a mixture of azure, methylene blue and eosin stain. It is specific for the phosphate groups of DNA and binds itself where there is a high amount of adenine-thymine bonding. Azur and eosin are acidic dyes that variably stain the basic components of cells such as cytoplasm, granules, etc. Methylene blue, on the other hand, acts as a basic stain for acidic components, especially the cell nucleus.
Warnings, Precautions, Recommendations
Highly flammable liquid and vapour. Toxic if swallowed, in contact with skin or inhaled. Causes damage to organs.
Ingredients
Giemsa stain, Methanol and Glycerol.
Colour
Odour
Storage Temperature
15/25°C
Shelf Life After Opening the Lid
3 months
Shelf Life
24 months
pH (at 25°C)
Appearance
Concentration
Giemsa stain, nucleic acid stain, blood film stain, Erythrocytes stain, lymphocyte cytoplasm stain, monocyte cytoplasm stain, leukocyte nuclear chromatin stain, peripheral blood smears
Accessories
Application
Staining of Smears
1. Preparation of Dye Solution (Dilute Giemsa Solution):
▪ Take 5 ml of GBL-2408 "Giemsa Stain; Concentrate" into the staining container.
Add 45 ml of distilled water and mix well.
2. Staining with Giemsa Solution:
▪ Cover the preparation with methanol and fix for 5 minutes.
Immerse the preparation in the prepared staining solution.
After 15-30 minutes, remove the slide and shake to remove the reagent.
Wash with distilled water. Dry it.
Examine under a microscope.
Microbiological Properties
Smear Staining Results:
Nucleus
Red/purple
Lymphocytes
Blue
Monocytes
Pale blue
Neutrophilic granulocytes
Granules light purple
Eosinophilic granulocytes
Red/grey blue
Basophilic granulocytes
Dark purple
Thrombocytes
Purple
Erythrocytes
Reddish
Blood parasites
Nucleus light red
Staining Results of Paraffin Sections:
Cell nucleus, cells
Blue, dark blue
Collagen, osteoid
Pale blue
Eosinophilic granules
Red
Acidophilic mucopolysaccharides
Reddish purple
Acidophilic materials
Orange red
Ordering and Packaging Information
Order No
Unit
Volume
Packaging Type
Quantity in a Parcel
442408050250
Bottle
250 ml
250 ml, PP White Bottle with Dropper
32
442408020500
Bottle
500 ml
500 ml, HDPE White Bottle
24
442408011000
Bottle
1000 ml
1000 ml, HDPE Brown Bottle
20
export@gbl.com.tr
+90 533 775 75 58
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